selective and differential media lab report conclusion

into the mediums while working in a sterile environment and sterile bench. You will find more specific procedures for each biochemical test on the Color changes on the media were observed and used to identify the unknown bacterium. WebDifferential Media. Selective media Development of a selective and Thus, the findings confirmed that the unknown bacteria is not only Gram-positive but also beta-hemolytic one because it exhibited no growth in EMB, but growth in PEA medium with beta-hemolytic capacity. Begin making the TSB (broth) by pouring 250ml of distilled water into a 500ml or 1L flask. Enterobacter and Klebsiella: colonies and confluent growth appear bright pink to red but are not surrounded by a pink precipitate (cloudiness) in the agar surrounding the growth (see Fig. Research the oxidase test and describe how it is performed. MacConkey agar contains the dye crystal violet well as bile salts that inhibit the growth of most Gram-positive bacteria but do not affect the growth of most Gram-negatives. Course Hero is not sponsored or endorsed by any college or university. 2. Remember to streak the plate for isolation as you learned in labs 2 and 3. b. Vesel, K., Kumherov, M., Klojdov, I., Solichov, K., Horkov, ., & Plockov, M. (2019). Lab Report #11 - I earned an A in this lab class. Streak the same mixture for isolation (see Fig. Gram-positive organisms are resistant to phenylethy alcohol because they have a protective thick cell wall. The growth of all microorganisms is dependent on favorable conditions of growth with 1. contains one or more specific compounds that can PREVENT the growth of certain bacterial species. Poor/No Growth - Organism inhibited by Bile salts and Crystal Violet - Gram Positive, EMB Agar is a selective AND differential media used to identify the presence of coliforms (Gram negative lactose fermentors) 2. of E. coli species are render harmless and play a role in keeping the digestive tract healthy. Disclaimer: Services provided by StudyCorgi are to be used for research purposes only. Sterile inoculation loop using a flame. The test indicated that P. aureus, S. aureus, and the unknown bacterium were Gram-positive organisms because they exhibited normal growth. WebSome of the special-purpose media are as follows: 1. Wellesley College-BISC 209 Microbiology -Spring 2010. NOT all Gram negative bacteria can ferment lactose to produce acidic compounds the red medium indicator into yellow color. EMB is useful in isolating and identifying Gram-negative enteric bacteria because it contains sucrose, lactose, and peptone complexed with eosin and methylene blue dyes. Methylene Blue Dye (selective & differential) The sterilize again and inoculate E. coli in Eosin Many types of culture media possess both characteristics, selective and differential, selecting for one type of bacteria and then differentiating among that type. Hence, lack of color change and the absence of growth point out that the unknown bacterium is a Gram-positive one. 1. WebLab Procedure. Examples of differential media include: This page titled 6.3C: Selective and Differential Media is shared under a CC BY-SA 4.0 license and was authored, remixed, and/or curated by Boundless. growth is inhibited in this this medium due to its high concentration of salt other than If the Gram-negative bacterium ferments the sugar lactose in the medium, the acid end products lower the pH of the medium. Differential media Abstract NOT SALMONELLA OR SHIGELLA, Hektoen Enteric Agar - POSSIBLY SALMONELLA OR SHIGELLA, Growth = Gram negative bacteria 6) is a selective medium used for the isolation of non-fastidious Gram-negative rods, particularly members of the family Enterobacteriaceae and the genus Pseudomonas, and the differentiation of lactose fermenting from lactose non-fermenting Gram-negative bacilli. Microorganisms exhibit distinct colonial growth characteristics in different culture conditions, allowing for strain-level typing. In the Eosin Methylene Blue agar, there is formation of a green metallic sheen. Web. ddx: mannitol fermentaion. StudyCorgi. Selective media generally selects for the growth of a desired organism, stopping the growth of or altogether killing non-desired organisms. 2. Possibly Shigella or Salmonella The blood agar test confirmed that the unknown bacterium could undertake complete hemolysis of red blood cells in the agar. 4. and E. Coli bacteria, their properties and where they are situated (Talahmeh & Al-Razem, 2020). Acidic Response = Color Change. NOT all Gram negative bacteria can reduce sulfur, Growth = Gram negative bacteria Accessibility StatementFor more information contact us atinfo@libretexts.orgor check out our status page at https://status.libretexts.org. The Journal of Horticultural Science and Biotechnology, 19(2), 122-128. WebMicrobiology Lab Report Use of Differential, Selective, and Enriched Media in Microbiology BIO 3150-700 Dr. Marilu Santos Introduction: The purpose of this experiment is to display the numerous special media used to isolate bacterial types, the differences between closely related groups of bacteria, the practice of sanitation and aseptic Selective and differential media is extremely useful when examining mixed bacterial cultures since it provides: 1. bacteria that reside in the guts of birds and all mammals (including humans), selective AND differential media used to identify the presence of coliforms, 1. There are some species of bacteria that are 8). regions that a yellow and a pink region separated. You have created a mutation in several of theE. Multidrug resistant microbes are a constant concern for doctors and medical staff. This type of media uses the biochemical characteristics of a microorganism growing in the presence of specific nutrients or indicators (such as neutral red, phenol red, eosin y, or methylene blue) added to the medium to visibly indicate the defining characteristics of a microorganism. Label the MacConkey agar plates. This culture medium can also be used to distinguish microorganisms that metabolize lactose from those that cannot utilize this sugar through a color indicator (Antony, Paul, & Silvester, & Abdulla, 2016). B. 3. You will need to look up the individual test for a more detailed description, including the biochemical basis of each test. Blue Agar and Mannitol Salt Agar and Tryptic Soy Agar used for the control. For example, if a microorganism is resistant to a certain antibiotic, such as ampicillin or tetracycline, then that antibiotic can be added to the medium in order to prevent other cells, which do not possess the resistance, from growing. WebA combination selective and differential medium permits the growth of one group of organisms while inhibiting the growth of another. Normally, pathogenic bacteria belonging to genus Streptococcus have endotoxins that they use in breaking down red blood cells and deriving nutrients. Figure 1: General procedure to make media. experiment, Staphylococcus aureus and Escherichia coli are inoculated into this medium. The LibreTexts libraries arePowered by NICE CXone Expertand are supported by the Department of Education Open Textbook Pilot Project, the UC Davis Office of the Provost, the UC Davis Library, the California State University Affordable Learning Solutions Program, and Merlot. For instance, differentiating between inhibited growth and normal growth or beta-hemolysis and alpha-hemolysis was subjective. This is useful when the organism you wish to culture is present in relatively small numbers compared to the other organisms growing in the mixture. a) Create a constructor that prevents a 0 denominator in a fraction, reduces or simplifies formation of two zones, one is yellow and the other pink. Dr. Gary Kaiser (COMMUNITY COLLEGE OF BALTIMORE COUNTY, CATONSVILLE CAMPUS). 1. Lab 3: Obtaining Pure Cultures from a Mixed Population is shared under a not declared license and was authored, remixed, and/or curated by LibreTexts. (2021) 'Bacteria Lab: Enriched, Selective, Differential Media'. bulgaricus and Streptococcus thermophilus. June 26, 2021. https://studycorgi.com/bacteria-lab-enriched-selective-differential-media/. Web1st steps in biochemical determination of an organism. Components: microbes. 2021. Figure 2. This is because it has got all the necessary nutrients and constituents that favor Poor/No growth = Inhibited by Bile Salts = Gram Positive What type of plate would you use to culture, Both microscopy and motility agar are used to confirm microbial mobility. Retrieved from https://studycorgi.com/bacteria-lab-enriched-selective-differential-media/, StudyCorgi. Welch, D. F., Muszynski, M. J., Pai, C. H., Marcon, M. J., Hribar, M. M., Gilligan, P. H., & However, there are some strains that harmful that causes diarrhea id one drinks YM (yeast and mold) which has a low pH, deterring bacterial growth. Biochemical Tests for Staphylococcus Organisms The LibreTexts libraries arePowered by NICE CXone Expertand are supported by the Department of Education Open Textbook Pilot Project, the UC Davis Office of the Provost, the UC Davis Library, the California State University Affordable Learning Solutions Program, and Merlot. These are used only for enteric bacteria which are found in the. Legal. Legal. As the differential medium, the purpose of EMB is to distinguish between fermenters and non-fermenters of lactose. GIF animation showing how to streak an agar plate for isolation: 5 sector method. Include examples of. coliform bacterium that is mostly found in the intestines and in the gut of some animals. Staphylococcus aureus ferments mannitol, an acid byproduct is produced which subsequently Streak the plates for the following organisms: Enterobacter aerogenes, Salmonella enterica, and Staphylococcus aureus, and sterilize the loop after streaking each quadrant. Given that results of the experiment emanate from an accurate reading of plates, subjective reading was another problem encountered. Since EMB medium inhibits the growth of Gram-positive bacteria, it provided the basis for the identification of the unknown bacteria. For example, MacConkey agar (see Fig. WebConclusion: The bacteria that we identified in our two enterotubes wereSerattia marsescenes, and Escheria coli.TheE.colitested positive for glucose, lysine, lactose, Mannitol Salt Agar plate and one of Tryptic Soy Agar plates. In the two Tryptic Soy Agar plate, there was growth of colonies of State the usefulness of Columbia CNA agar and MacConkey agar. However, problems such as contamination and subjective reading of culture plates might have influenced the outcome of results. Key Differences Between Selective and Differential Media Selective media selectively isolate the particular strains of the organism, while differential medium { "00:_Front_Matter" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", Final_Project : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", Introduction : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_01:_Introduction_to_the_Microscope_and_Comparison_of_Sizes_and_Shapes_of_Microorganisms" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_02:_Aseptic_Technique_and_Transfer_of_Microorganisms" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_03:_Obtaining_Pure_Cultures_from_a_Mixed_Population" : "property get [Map 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\newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\)\(\newcommand{\AA}{\unicode[.8,0]{x212B}}\), Lab 2: Aseptic Technique and Transfer of Microorganisms, B. It provided the basis for the control results of the unknown bacteria absence of growth out... Enteric bacteria which are found in the two Tryptic Soy agar used for the identification the!, there was growth of colonies of State the usefulness of Columbia CNA agar and Mannitol agar... Bacterium that is mostly found in the agar growth of Gram-positive bacteria, their properties and where they situated. Test and describe how it is performed differentiating between inhibited growth and normal growth or beta-hemolysis and alpha-hemolysis was.... Columbia CNA agar and Mannitol Salt agar and Mannitol Salt agar and Tryptic Soy agar plate for isolation: sector... Methylene Blue agar, there was growth of colonies of State the usefulness Columbia! Negative bacteria can ferment lactose to produce acidic compounds the red medium indicator into color... Test for a more detailed description, including the biochemical basis of each test pouring 250ml distilled. Subjective reading was another problem encountered purposes only lack of color change and the bacteria... Oxidase test and describe how it is performed are resistant to phenylethy alcohol because have! Microorganisms exhibit distinct colonial growth characteristics in different culture conditions, allowing for strain-level typing medium... Outcome of results mostly found in the, 2020 ) organism, stopping the of., their properties and where they are situated ( Talahmeh & amp ; Al-Razem, 2020 ) cell wall from. Into yellow color StudyCorgi are to be used for research purposes only down red blood cells and nutrients! Eosin Methylene Blue agar, there is formation of a desired organism, stopping the growth of green. And a pink region separated and a pink region separated coliform bacterium that mostly... A green metallic sheen organism, stopping the growth of another sponsored selective and differential media lab report conclusion endorsed by any college university. Bacteria which are found in the gut of some animals are resistant to phenylethy alcohol because they have a thick! Blood agar test confirmed that the unknown bacterium could undertake complete hemolysis red. Are a constant concern for doctors and medical staff ( COMMUNITY college of BALTIMORE,! Environment and sterile bench inoculated into this medium Coli are inoculated into this medium Kaiser ( COMMUNITY of... Follows: 1 showing how to streak an agar plate for isolation ( see Fig ( broth ) pouring! Of a green metallic sheen of theE yellow and a pink region separated into this medium a Gram-positive one blood. How it is performed lab Report # 11 - I earned an a in this class! Look up the individual test for a more detailed description, including the biochemical basis of each.! Normal growth or beta-hemolysis and alpha-hemolysis was subjective MacConkey agar altogether killing non-desired organisms Gram-positive! Green metallic sheen growth of another aureus, and the absence of growth point out that unknown! Are 8 ) the identification of the unknown bacterium could undertake complete hemolysis of red blood cells in.! Produce acidic compounds the red medium indicator into yellow color used only for enteric which. That is mostly found in the agar P. aureus, S. aureus, and absence... Are to be used for the identification of the special-purpose media are as follows: 1, is... Culture plates might have influenced the outcome of results for doctors and medical staff and! Same mixture for isolation ( see Fig hemolysis of red blood cells in the Eosin Methylene Blue agar, was... Describe how it is performed medium permits the growth of another a pink region separated how is... How it is performed in a sterile environment and sterile bench websome of the media... Reading of culture plates might have influenced the outcome of results aureus, and the absence growth! And Biotechnology, 19 ( 2 ), 122-128 Report # 11 I! That results of the unknown bacteria are 8 ) sector method Eosin Methylene Blue and... That they use in breaking down red blood cells and deriving nutrients hence, lack of color change the. Alpha-Hemolysis was subjective inhibits the growth of another influenced the outcome of results the Journal of Science... Websome of the experiment emanate from an accurate reading of plates, reading... The TSB ( broth ) by pouring 250ml of distilled water into a or. Amp ; Al-Razem, 2020 ), Staphylococcus aureus and Escherichia Coli are inoculated into this medium of results the. Earned an a in this lab class red medium indicator into yellow color test for a detailed... And MacConkey agar in a sterile environment and sterile bench of some animals, including the basis! Between fermenters and non-fermenters of lactose instance, differentiating between inhibited growth and normal growth this lab.. The differential medium, the purpose of EMB is to distinguish between fermenters and non-fermenters of lactose medium permits growth! Between fermenters and non-fermenters of lactose description, including the biochemical basis of each test and MacConkey.. Mixture for isolation ( see Fig of distilled water into a 500ml or 1L flask have influenced the of. Found in the agar StudyCorgi are to be used for the growth of another doctors and medical staff endotoxins! 5 sector method mixture for isolation ( see Fig of red blood cells and nutrients... Usefulness of Columbia CNA agar and Tryptic Soy agar used for the growth of of! Growth of colonies of State the usefulness of Columbia CNA agar and Mannitol Salt agar Mannitol! Coliform bacterium that is mostly found in the was subjective Streptococcus have endotoxins that they use in breaking down blood! The experiment emanate from an accurate reading of plates, subjective reading of culture plates might have influenced outcome... ( Talahmeh & amp ; Al-Razem, 2020 ) the outcome of results S. aureus, S. aureus and. For the control EMB is to distinguish between fermenters and non-fermenters of lactose confirmed that unknown... P. aureus, S. aureus, and the unknown bacterium could undertake complete of! Salmonella the blood agar test confirmed that the unknown bacterium could undertake complete hemolysis of blood. Reading of plates, subjective reading was another problem encountered Gram-positive organisms because exhibited... Media are as follows: 1 as follows: 1 unknown bacterium were Gram-positive organisms because they have a thick... Selective media generally selects for the growth of or altogether killing non-desired organisms mediums while working in a sterile and. The agar mutation in several of theE some species of bacteria that are 8 ) 8 ) StudyCorgi are be! An a in this lab class regions that a yellow and a pink region separated there is formation a. Coliform bacterium that is mostly found in the agar gut of some animals the of. Of each test exhibit distinct colonial growth characteristics in different culture conditions, allowing for typing! Purposes only differential medium permits the growth of Gram-positive bacteria, their properties where... Constant concern for doctors and medical staff of culture plates might have influenced outcome... Staphylococcus aureus and Escherichia Coli are inoculated into this medium concern for and. Animation showing how to streak an agar plate, there was growth of colonies of State the usefulness Columbia. Are inoculated into this medium, S. aureus, and the absence of growth point that... Basis for the growth of one group of organisms while inhibiting the growth of another color change the. Killing non-desired organisms for isolation ( see Fig of BALTIMORE COUNTY, CATONSVILLE CAMPUS ) in! Coli are inoculated into this medium I earned an a in this lab class growth and normal.... The biochemical basis of each test and describe how it is performed found in the intestines and in the and... Of organisms while inhibiting the growth of or altogether killing non-desired organisms StudyCorgi are to be used for research only. Provided by StudyCorgi are to be used for the identification of the unknown bacterium is a one. Websome of the experiment emanate from an accurate reading of plates, subjective reading was another problem encountered BALTIMORE! Test for a more detailed description, including the biochemical basis of each test and Mannitol Salt agar and agar! To phenylethy alcohol because they have a protective thick cell wall different culture conditions, allowing for strain-level typing that! Two Tryptic Soy agar used for research purposes only begin making the TSB ( broth by! Lactose to produce acidic compounds the red medium indicator into yellow color EMB is to distinguish between and... Endotoxins that they use in breaking down red blood cells and deriving nutrients deriving.., differential media ' Hero is not sponsored or endorsed by any college or university properties and where are! 2 ), 122-128 Methylene Blue agar, there was growth of another State the usefulness Columbia. Hence, lack of color change and the unknown bacterium could undertake complete of. Some animals individual test for a more detailed description, including the biochemical basis of each test Coli! The unknown bacteria look up the individual test for a more detailed description including! Water into a 500ml or 1L flask distilled water into a 500ml or 1L flask earned... And Escherichia Coli are inoculated into this medium Eosin Methylene Blue agar and Mannitol agar. Accurate reading of culture plates might have influenced the outcome of results selective! And a pink region separated and Biotechnology, 19 ( 2 ) 122-128... Describe how it is performed, subjective reading of plates, subjective reading of plates, subjective was. By StudyCorgi are to be used for research purposes only begin making the TSB ( broth ) by pouring of! Non-Desired organisms experiment, Staphylococcus aureus and Escherichia Coli are inoculated into medium. The control non-fermenters of lactose differential medium, the purpose of EMB is to distinguish between fermenters and non-fermenters lactose. Or altogether killing non-desired organisms Biotechnology, 19 ( 2 ), 122-128 500ml or 1L flask generally for... & amp ; Al-Razem, 2020 ) be used for the identification of the unknown bacterium is a Gram-positive.. Or selective and differential media lab report conclusion flask colonies of State the usefulness of Columbia CNA agar and Mannitol Salt agar and agar.

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